Table II.
qPCR was performed to confirm the results from the DNA microarray assay.
| McA-RH7777 | McA-RH7777 + iHSC | |||||
|---|---|---|---|---|---|---|
|
|
|
|||||
| Gene | Abbreviation | Exp. 1 | Exp. 2 | Exp. 1 | Exp. 2 | log2 iHSCc |
| TAP binding protein | Tapbp | 9.609 | 9.868 | 11.842 | 11.797 | 2.081 |
| Intercellular adhesion molecule 1 | Icam1 | 12.916 | 12.918 | 14.14 | 14.053 | 1.1795 |
| Chemokine (C-X-C motif) ligand 1a | Cxcl1 | 13.161 | 13.185 | 14.45 | 14.499 | 1.3015 |
| Chemokine (C-C motif) ligand 2 | Ccl2 | 4.617 | 4.38 | 6.42 | 6.411 | 1.917 |
| Chemokine (C-X-C motif) ligand 10 | Cxcl10 | 12.13 | 12.008 | 15.67 | 15.741 | 3.6365 |
| Jun B proto-oncogene | Junb | 9.242 | 9.274 | 10.43 | 10.415 | 1.1645 |
| Interferon regulatory factor 7 | Irf7 | 7.439 | 8.38 | 15.506 | 15.499 | 7.593 |
| Interferon regulatory factor 9 | Irf9 | 8.061 | 8.32 | 10.499 | 10.497 | 2.3075 |
| Interferon regulatory factor 1 | Irf1 | 6.754 | 7.285 | 9.867 | 9.843 | 2.8355 |
| Septin 4 | 4-Sep | 8.874 | 8.854 | 7.771 | 7.7 | −1.1285 |
| Platelet-derived growth factor receptorb | Pdgfra | 6.51 | 7.079 | 5.499 | 5.458 | −1.316 |
| Integrin, alpha E | Itgae | 11.244 | 11.021 | 10.081 | 10.021 | −1.0815 |
a
Melanoma growth stimulating activity, alpha;
b
alpha polypeptide;
c
vs. Control. Compared with McA-RH7777 cells cultured alone, Itgae expression was down-regulated, and the expression of Irf1 and Irf9 was up-regulated in McA-RH7777 cells co-cultured with iHSCs.
qPCR, quantitative PCR; Exp, experiment.