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. 2014 Aug 28;9(8):e105932. doi: 10.1371/journal.pone.0105932

Table 1. Primer sequences.

Primer name 1Sequence (5′-3′)
Primers for SGC cloning
LIR241 PacI (F) TTAATTAA GCCGACGACGGAGGTTGAGG
LIR241 SwaI (R) ATTTAAAT CATACAAAGCAGAACCAGGC
GUSex1BamHI (F) GGATCC ATGGTACGTCCTGTAGAAACCCCAACCCG
GUSex1 (R) GAGTTTCATCGTACGGTACTTGAG
GUSex2 (F) GTGCGCCGTAGTTTCCTTTAG
GUSex2SpeI (R) ACTAGT TTATTGGAGATCCTCATTGTTTGC
UbiAscI (F) GGCGCGCC AAGCTTGCATGCCTGCAGTGCAG
UbiBamHI (R) GGATCC TCTAGAGTCGACCTG
UbiΔIBamHI (R) GGATCC AGAGGGTGTGGAGGGGGTGTCTATTTATTACG
Real-time PCR Primers
MSV-Kom Rep (F) TTGGCTGTCAGAGGGATTTC
MSV-Kom Rep (R) CCCTGGAGTCATTTCCTTCA
MSV-Kom CP (F) TAAGCGGGTGCCTAAGAAGA
MSV-Kom CP (R) TGCTGGAGTGTCTGGATTTG
MSV-VW CP (F) GGGAGATGATTCGAACTGGA
MSV-VW CP (R) TGCTGGAGTGTCTGGATCTG
MSV-Set CP (F) AGTTGTGTCATCGCTTCGTG
MSV-Set CP (R) TGGTGTATCCGAGCCTATCC
PanSV-Kar CP (F) CCACACCAACGAGACTCTGA
PanSV-Kar CP (R) CAACCACATGACACCCACTC
Maize18S (F) CAGGGATCAGCGGTGTTACT
Maize 18S (R) GGTAAGTTTCCCCGTGTTGA
1

Underlined letters highlight engineered restriction enzyme (RE) sites (names of the introduced RE sites are incorporated in the primer names).