Table 1. Primer sequences.
Primer name | 1Sequence (5′-3′) |
Primers for SGC cloning | |
LIR241 PacI (F) | TTAATTAA GCCGACGACGGAGGTTGAGG |
LIR241 SwaI (R) | ATTTAAAT CATACAAAGCAGAACCAGGC |
GUSex1BamHI (F) | GGATCC ATGGTACGTCCTGTAGAAACCCCAACCCG |
GUSex1 (R) | GAGTTTCATCGTACGGTACTTGAG |
GUSex2 (F) | GTGCGCCGTAGTTTCCTTTAG |
GUSex2SpeI (R) | ACTAGT TTATTGGAGATCCTCATTGTTTGC |
UbiAscI (F) | GGCGCGCC AAGCTTGCATGCCTGCAGTGCAG |
UbiBamHI (R) | GGATCC TCTAGAGTCGACCTG |
UbiΔIBamHI (R) | GGATCC AGAGGGTGTGGAGGGGGTGTCTATTTATTACG |
Real-time PCR Primers | |
MSV-Kom Rep (F) | TTGGCTGTCAGAGGGATTTC |
MSV-Kom Rep (R) | CCCTGGAGTCATTTCCTTCA |
MSV-Kom CP (F) | TAAGCGGGTGCCTAAGAAGA |
MSV-Kom CP (R) | TGCTGGAGTGTCTGGATTTG |
MSV-VW CP (F) | GGGAGATGATTCGAACTGGA |
MSV-VW CP (R) | TGCTGGAGTGTCTGGATCTG |
MSV-Set CP (F) | AGTTGTGTCATCGCTTCGTG |
MSV-Set CP (R) | TGGTGTATCCGAGCCTATCC |
PanSV-Kar CP (F) | CCACACCAACGAGACTCTGA |
PanSV-Kar CP (R) | CAACCACATGACACCCACTC |
Maize18S (F) | CAGGGATCAGCGGTGTTACT |
Maize 18S (R) | GGTAAGTTTCCCCGTGTTGA |
Underlined letters highlight engineered restriction enzyme (RE) sites (names of the introduced RE sites are incorporated in the primer names).