Table 3. Virus-host and virus-virus DNA junctions detected in livers and lymphoid tissues and cells of woodchucks with lifelong primary occult WHV infection.
Subgroup/Animal no. | Tissue type | Time of sample acquisition (m.p.i.) detected no. | Overall virus-host DNA junction detected no. | Characteristics of virus-host DNA junction | Overall virus-virus junction | ||
WHV sequence* | Host sequence length (bp) | Host sequence identity | |||||
With HCC | |||||||
5/M | Liver autopsy | 55 | 2 | S374-380 | 36 | n.d. | 2 |
S301-306 | 236 | n.d. | |||||
Bone marrow | 55 | 2 | S575-569 | 272 | n.d. | 6 | |
X1590-1583 | 170 | n.d. | |||||
Liver biopsy | 32 | PS132-138 | 263 | n.d. | 2 | ||
Liver biopsy | 20 | 2 | X1877-1916 | 69 | n.d. | 0 | |
P1338-1441 | 59 | n.d. | |||||
9/M | Liver autopsy | 55 | 3 | X1782-1808 | 246 | n.d. | 6 |
X1782-1810 | 264 | H19; mouse 80% | |||||
P1149-1156 | 276 | n.d. | |||||
Bone marrow | 55 | 1 | X1513-1627 | 83 | n.d. | 0 | |
Liver biopsy | 46 | 2 | X1674-1386 | 82 | n.d. | 0 | |
X1632-1317 | 35 | n.d. | |||||
Liver biopsy | 32 | 1 | X1898-2060 | 66 | n.d. | 0 | |
Without HCC | |||||||
1/F | Bone marrow | 66 | 1 | P1382-C2335 | 241 | n.d. | 4 |
PBMC | 66 | 1 | X1854-1861 | 198 | n.d. | 2 | |
2/F | Lymph node | 34 | 4 | C2039-2044 | 211 | n.d. | 6 |
P1479-1491 | 146 | n.d. | |||||
S351-343 | 86 | n.d. | |||||
PS56-61 | 78 | n.d. | |||||
8/M | Liver autopsy | 22 | 1 | X1636-P1343 | 219 | n.d. | 0 |
Bone marrow | 22 | 2 | X1636-1501 | 177 | n.d. | 0 | |
X1853-1915 | 123 | n.d. | 0 | ||||
10/M | Liver autopsy | 72 | 1 | X1627-P1151 | 39 | n.d. | 0 |
Bone marrow | 72 | 2 | X1636-P1088 | 88 | n.d. | 0 | |
X1616-1672 | 130 | n.d. | 0 |
* WHV X, S, P gene or preS (PS) genomic region sequence flanking virus-host junction.
Abbreviations: m.p.i., months post-infection; no., number; n.d., not determined; n.a., not applicable.