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. 2014 Aug 28;9(8):e106369. doi: 10.1371/journal.pone.0106369

Figure 4. The resolution of R-PC and AP in Bis-tris-HEPES-MES buffer system with the stacking gel.

Figure 4

The PAGE with the stacking gel of 4% in pH 6.0 Bis-tris−HEPES−Acetic acid buffer was performed respectively with: the resolving gel of 7% (A) and of 9.5% (B) in pH 7.0 (A) and pH 6.5 (B) Bis-tris−HEPES−MES buffers; the Tricine−Bis-tris electrode buffers in pH 6.5 (A) and pH 6.3 (B) were used in the PAGE. (a) the bands in natural colors, (b) the bands of native state in grey scale without red filter, (c) the bands of native state with red filter, (d) the bands in fluorescence under UV-light at 365 nm and (e) the bands stained by Coomassie Blue G-250. The sample used in the PAGE was the R-PC and AP fraction obtained by the ion exchange chromatography.