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. 2014 Aug 28;9(8):e106369. doi: 10.1371/journal.pone.0106369

Figure 5. The resolution of AP in Bis-tris-HEPES-MES buffer system without stacking gel.

Figure 5

The PAGE was carried out with a resolving gel of 9% in pH 6.5 Bis-tris−HEPES−MES buffer and with no stacking gel exclusively for the AP preparation. (a) the bands in natural colors, (b) the bands of native state in grey scale without red filter, (c) the bands of native state in grey scale with red filter, (d) the bands in fluorescence under UV-light at 365 nm and (e) the bands stained by Coomassie Blue G-250. The Tricine−Bis-tris electrode buffers in pH 6.3 were used in the PAGE. The sample used in the PAGE was the R-PC and AP fraction from the ion exchange chromatography.