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. 2014 Aug 28;10(8):e1004334. doi: 10.1371/journal.ppat.1004334

Figure 6. ESI mass spectra to examine polymannuronic acid binding.

Figure 6

ESI mass spectra acquired for aqueous ammonium acetate (50 mM) solutions of (A) PaAlgX27–474 (P) (4 µM) and 50 mM ManA6); (B) PaAlgX27–474(P) (4 µM) and 50 mM ManA12); (C) PaAlgJ79–379 (P) (10 µM) and 50 mM ManA6); (D) PaAlgJ79–379 (P) (10 µM) and 50 mM ManA12). A reference protein scFv (4 µM) was added into the solutions of AlgX, Lyz was added as Pref to AlgJ solutions. Ion peaks corresponding to higher charge states of AlgJ are labelled as filled red circles. Pref refers to the reference protein scFv.