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. 2014 Aug 28;10(8):e1004334. doi: 10.1371/journal.ppat.1004334

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© 2014 Baker et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Mass spectra of the acetyltransferase reactions in the presence (red, line a) and absence (blue, line b) of PaAlgX_27–474. Major peaks are labelled: ManA10, mannuronic acid decamer; ManA10+Na, mannuronic acid decamer plus sodium; ManA10+OAc, mannuronic acid decamer plus acetate; ManA10+Na+OAc, mannuronic acid decamer plus sodium and acetate; ManA10+2 OAc, mannuronic acid decamer plus two acetates. The observed molecular weights of each product are denoted under their respective labels. The addition of acetate adds 42.01 Da to the mass of the polymer. The data has been deconvoluted and centroided using Agilent MassHunter software and represents uncharged exact masses.