Table 3. Apparent association constants (K a) for PaAlgX27–474 and PaAlgJ79–379 for short polymannuronic oligosaccharides at 298 K and pH 7 determined by the direct ESI-MS assay.
Length of oligosaccharide (Ligand name)* | Apparent K a (M−1) AlgX | Apparent K a (M−1) AlgJ |
4 (ManA4) | (1.0±0.5)×103 | <500 |
5 (ManA5) | (1.0±0.5)×103 | <500 |
6 (ManA6) | (3.2±0.3)×103 | <500 |
7 (ManA7) | (4.8±1.0)×103 | <500 |
8 (ManA8) | (5.8±1.0)×103 | NB |
9 (ManA9) | (8.8±1.0)×103 | NB |
10 (ManA10) | (10.0±0.7)×103 | NB |
11(ManA11) | (16.0±0.5)×103 | NB |
12 (ManA12) | (19.0±0.3)×103 | NB |
NB: No Binding.
*Ligand name as referenced in [78].