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. 2014 Aug 28;10(8):e1004346. doi: 10.1371/journal.ppat.1004346

Figure 6. Survival of different Y. pseudotuberculosis strains in macrophages as determined by fluorescence microscopy.

Figure 6

(A) BMDMs were infected with the indicated strains at an MOI of 10 as described in Figure 1. At 2 h and 24 h post infection, infected cells were fixed, permeabilized and labeled with a rabbit anti-Yersinia antibody (green). Shown is bacteria or an overlay of bacteria and phase contrast signal from representative images. (B) Percentage of macrophages containing fluorescent Yersinia at 24 h post infection, quantified from three independent microscopy experiments as described in (A). Error bars show standard deviations. ***, P<0.001 compared to IP6+YopE, determined by one-way ANOVA.