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. 2014 Aug 28;10(8):e1004348. doi: 10.1371/journal.ppat.1004348

Figure 1. A microfluidic system for tracking retrograde transport in sensory axons.

Figure 1

(A) A Polydimethylsiloxane (PDMS) microfluidic chamber used for explant culture. (B,C) 40 µl interval towards the proximal compartment (top) prevents fluorescent dye from diffusing to the proximal compartment, allowing several hours of compartmental separation. Bright field (B) and fluorescent images (C) taken 7.5 hours after addition of Sulforhodamine B fluorescent dye to the distal compartment (bottom). (D) DRG explants are healthy and extend axons through micro-grooves to distal compartment after 2–3 days in vitro. One microgroove typically contains 2–5 axons. Mosaic of 10× images of Calcein-stained DRG explant taken after 5 DIV. Scale bar = 50 µm.