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. 2014 Aug 28;10(8):e1004348. doi: 10.1371/journal.ppat.1004348

Figure 2. Rabies virus retrograde transport in DRG is faster and more directed than that of NGF.

Figure 2

Retrograde transport of (A) EGFP-RABV and (B) Quantum-dot conjugated NGF in DRG explants, roughly 2 hours after addition to distal axon compartment. Arrows and arrowheads pointing at transported particles. Scale bars = 10 µm. (C,D) Kymograph for EGFP-RABV and NGF respectively. Horizontal scale bars = 5 µm, vertical scale bars = 40 seconds. (E–J) Characterization of manually tracked directed particles of RABV (n = 209) and NGF (n = 149). (E) RABV is transported faster than NGF, as seen by average speed. (F) RABV is more directed and pauses less than NGF (G) Average pause duration was not significantly different. (H) RABV spent a smaller fraction of its run paused (I) Instantaneous velocities of RABV particles (n = 9885 events) are higher than those of NGF particles (n = 8973 events). Positive and negative values represent retrograde and anterograde velocities, respectively. (J) RABV particles travel larger net distances than NGF's, as seen by mean squared displacements. Data was pulled from two separate experiments. Error bars represent SEM, *p<0.0001.