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. 2014 Aug 28;10(8):e1004348. doi: 10.1371/journal.ppat.1004348

Figure 3. RABV and NGF present similar internalization kinetics at the axon tip.

Figure 3

Live TIRF microscopy was used to track RABV and NGF internalization in DRG neuron tips. (A) EGFP-RABV (dashed circles) is detected on the surface of a neuron tip (white line). Lower panels present a particle (circled in black) disappearing gradually into the cell over a course of ∼9 seconds. (B) Qdot labeled NGF undergoes gradual internalization at the tip of the DRG neuron. (C) Average internalization time of RABV and NGF (n = 6 and 8, respectively), from onset of gradual signal reduction to its disappearance, do not differ significantly [p = 0.148]. (D) Representative time course of individual RABV (blue) and NGF (red) particles intensity profile, from detection on the cell surface to their internalization. Arrows represent direction of the neuron soma. Scale bars = 5 µm.