Fig. 1.

Mapping the interaction between Hsp20 and Aβ_1–42.

Peptide array was used to map the domains responsible for Hsp20/Aβ_1–42 interaction. (A) Diagram of domain structure of Hsp20 highlighting the PKA/PKG site located in the N-terminal domain and the conserved α-crystallin domain located between residues 70 and 144. (B) Peptide array libraries of Hsp20 25mers were probed with either Aβ_1–42 or Aβ_scr. (C) Alanine scanning arrays of peptide 3 (W¹¹–E³⁵) were probed with either Aβ_1–42 (middle panel) or Aβ_scr (upper panel) to determine the Hsp20 amino acids that are essential for Aβ_1–42 binding. The association of Aβ_1–42 with substitution arrays in which serine 16 was replaced by a phospho-serine or phospho-mimetic substitution (serine changed to aspartic acid) was also evaluated (lower panel). * = p < 0.05, ** = p < 0.01 using Student-t-test (n = 4).