Fig. 5.

Hsp20 over-expression attenuates Aβ_1–42 induced cytotoxicity in SH-SY5Y cells.

(A) A dose response curve of cell viability (as measured by cell index) was constructed over a range of Aβ_1–42 concentrations in SH-SY5Y cells transfected with empty vector or Hsp20. Relative levels of phospho-Hsp20 and total Hsp20 were determined by western blotting (left panels). Impedance growth profiles of SH-SY5Y cells transfected with empty vector, Hsp20Wt, Hsp20S16D, and Hsp20S16A (see inset for relative expression levels) were measured over 48 h following treatment with (B) Aβ_scr or (C) Aβ_1–42. Quantifications of cell index at 48 h compared with the scrambled control in (A) were determined (n = 3) and statistical evaluation undertaken * = p < 0.05 and *** = p < 0.001 using Student-t-test.