Table 1.

Results of methylation analysis

Population	Species	Lineage	Locus silenced	Methylated? a
Pullman	T. dubius	2613-1	NA
	T. dubius	2613-11	NA
	T. miscellus	2605-4	TDF44d	Yes-A/S
	T. miscellus	2605-7	TDF44d	Yes-A/S
	T. miscellus	2605-13	TDF44d	Yes-A/S
			S18d	Yes-A
	T. miscellus	2605-24	TDF44d	Yes-A/S
	T. miscellus	2605-28	TDF44d	Yes-A/S
	T. miscellus	2605-46	TDF44d	Yes-A/S
Moscow	T. pratensis	2608-31	NA
	T. pratensis	2608-35x	NA
	T. miscellus	2604-4	TDF44d	Yes-A/S
	T. miscellus	2604-11	TDF44d	Yes-A/S
	T. miscellus	2604-15	TDF44d	Yes-A/S
	T. miscellus	2604-22	*T. pratensis genomic copy lost
	T. miscellus	2604-24	TDF44d	Yes-A/S
	T. miscellus	2604-35	TDF44d	Yes-A/S
Spangle	T. miscellus	2693-7	S3p	No
	T. miscellus	2693-8	S3p	No
	T. miscellus	2693-14	S3p	No
			S18d	No
Garfield	T. dubius	2687-11	NA
	T. pratensis	2689-17	NA
	T. miscellus	2688-3	S2d	Yes-A/S
			S3d	No
			S18d	No
Oakesdale	T. miscellus	2671-11	S2d	Yes-A/S

NA = Not applicable; subscript d or p indicates the homeolog silenced; ^aA = antisense strand, S = sense strand.

Individual plants used in the study and their methylation status for the genes studied; silencing data from [42, 43].