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. 2014 Aug 13;12:78. doi: 10.1186/1477-7827-12-78

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© Araújo et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Isolated follicles by tissue chopper and microdissection, and in situ follicles. Isolated follicles (A) by tissue chopper and microdissection, and (B) in situ follicles stained with PAS-hematoxylin. o: oocyte; n: oocyte nucleus; fgc: flattened granulosa cells; cgc: cuboidal granulosa cells; tc: theca cells; zp: zona pellucida. A large secondary follicle and an antral follicle grown in vitro and isolated by microdissection are shown (A).