FIGURE 1. Scheme of the construction of rPreS fusion proteins for vaccination against birch pollen allergy.
(A) Amino acid sequence of Bet v 1a. Peptide PA (light gray box) and peptide PB (dark gray box), which are part of the recombinant fusion proteins, as well as Bet v 1–derived peptides P1′– P6′ used for epitope-mapping experiments, are indicated. (B) Illustration of the four rPreS fusion proteins (2PA-PreS, 2PB-PreS, 4PA-PreS, 2PAPB-PreS). Peptide A (PA: light gray), peptide B (PB: dark gray), the carrier protein PreS (white), and the C-terminal hexahistidine tags (6xHis: black) are indicated.