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. Author manuscript; available in PMC: 2014 Aug 29.
Published in final edited form as: J Immunol. 2013 Feb 25;190(7):3068–3078. doi: 10.4049/jimmunol.1202441

FIGURE 2. Characterization of recombinant fusion proteins.

FIGURE 2

(A) Coomassie-stained SDS-PAGE showing purified rPreS fusion proteins and rPreS. (B) Nitrocellulose-dotted PreS fusion proteins and PreS probed with rabbit anti-PreS Ig (lane 1), rabbit preimmune Ig (lane 2), or buffer alone (lane 3) were reacted with 125I-labeled goat anti-rabbit Ig. In addition, dotted proteins were probed with mAbs specific for Bet v 1–derived peptide P2′ (mAb2) (lane 4), Bet v 1–derived peptide P4′ (mAb12) (lane 5), or buffer alone (lane 6) and reacted with 125I-labeled rabbit anti-mouse Ig.

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