FIGURE 8. Inhibition of basophil activation in allergic patients by anti–2PAPB-PreS and anti–rBet v 1 Abs.

Increasing concentrations (0.0000132–0.01 μg/ml) of rBet v 1 in the presence of anti–2PAPB-PreS, anti–rBet v 1, or rabbit preimmune Ig were exposed to blood samples of three patients allergic to birch pollen [A13 (A), A22 (B), A24 (C)]. Anti-IgE Abs or buffer alone were used as controls (Co). Upregulation of CD203c expression is displayed as SI. Means of triplicate measurements are shown, and SD are indicated. The mean fluorescence intensity of unstimulated cells were as follows: A13: 126.3; A22: 127.7; A24: 99.3.