Fig. 7.

Double-label immunofluorescence microscopy of cross-sections through seminiferous tubules of frozen bull testis, showing the near-complete colocalization of N-cadherin (a, red, mouse mAb) and the plaque protein p0071 (a', green, guinea pig antibodies) in the AJs of the Sertoli cells, demonstrated by the yellow merger colour (a'', a'''; the latter is shown on a phase contrast background and with DAPI-staining of nuclei). Note also the intensive green immunostaining of the p0071 reaction in the junctions connecting endothelial cells in blood and lymph vessels (here indicated, e.g., by the arrow in the lower right-hand corner of a'–a'''). L, lumen. Bar 50 μm