Fig. 3.

(A) Undirected protein regulatory network in M. galloprovincialis. Pearson correlation coefficients are used to identify nodes, or proteins, that are similarly regulated and subsequently clustered. Temperature, highlighted in the shaded subnetwork, is shown to interact with P23 (gelsolin), which interacts with P13 (nucleoside diphosphate kinase), P18B (Cu-Zn superoxide dismutase), and P24A (actin). Positive correlations are shown with a solid line and negative correlations with a dashed line. The disconnected networks suggest that most proteins interact with a close neighborhood of proteins, rather than more globally across the network. (B) Directed protein regulatory network inferred using the GENIE3 algorithm. Directed networks illustrate information flow as described by the arrows among nodes. This network suggests that P21A (α-tubulin) and P6 (proteasome-α-type-1) are upstream regulators of P4B (α-crystalline-Hsp23).