Table 3. Cardiac expression of glucose and FA metabolism related genes measured by RT-PCR.
| pathway/metabolism | gene | Aase WT | Aase Vegf-b-/- | Bellomo WT | Bellomo Vegf-b-/- |
|---|---|---|---|---|---|
| glucose | Pdha1 | 2.729 ± 0.32 | 3.051 ± 0.16 | 3.231 ± 0.26 | 3.093 ± 0.30 |
| Pdk4 | 3.037 ± 0.39 | 3.737 ± 0.48 | 2.986 ± 0.31 | 2.422 ± 0.15 | |
| Glut4(Slc2a4) | 2.603 ± 0.28 | 3.064 ± 0.18 | 2.969 ± 0,32 | 3.005 ± 0.23 | |
| FA oxidation | Acadm | 1.144 ± 0.07 | 1.203 ± 0.16 | 0.977 ± 0.11 | 1.104 ± 0.08 |
| Acadl | 1.244 ± 0.12 | 1.293 ± 0.17 | 1.022 ± 0.12 | 1.181 ± 0.09 | |
| Cpt1β | 1.106 ± 0.08 | 1.321 ± 0.24 | 0.939 ± 0.14 | 1.023 ± 0.10 | |
| FA transport | Fatp3(Slc27a3) | 1.070 ± 0.06 | 1.304 ± 0.16 | 0.912 ± 0.12 | 0.926 ± 0.12 |
| Fatp4(Slc27a4) | 1.303 ± 0.11 | 1.445 ± 0.22 | 0.912 ± 0.10 | 1.015 ± 0.08 | |
| FA synthesis | Fasn | 1.110 ± 0.08 | 1.135 ± 0.15 | 0.891 ± 0.11 | 1.005 ± 0.08 |
| co-factors | Pgc-1α | 2.521 ± 0.45 | 3.026 ± 0.16 | 3.350 ± 0.36 | 2.954 ± 0.39 |
| Ncor1 | 2.497 ± 0.40 | 3.145 ± 0.26 | 3.496 ± 0.26 | 3.527 ± 0.58 | |
| Pparα | 2.545 ± 0.43 | 2.859 ± 0.12 | 3.192 ± 0.36 | 3.213 ± 0.26 |
Pdha1,pyruvate dehydrogenase alpha 1; Pdk4, pyruvate dehydrogenase kinase, isozyme 4; Slc2a4/Glut4, glucose transporter 4; Acadm, acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain; Acadl, acyl-Coenzyme A dehydrogenase, long chain; Cpt1β, carnitine palmitoyltransferase I beta; Slc27a3/Fatp3, fatty acid transport protein 3; Slc27a4/Fatp4, fatty acid transport protein 4; Fasn, fatty acid synthase; Pgc-1α, peroxisome proliferator-activated receptor gamma, coactivator 1 alpha; Ncor1, nuclear receptor co-repressor 1; Pparα, peroxisome proliferator-activator receptor alpha. Messenger RNA expression of indicated genes was normalized to B2M or PPIA. Results are expressed as mean ± S.E.M. n = 5–7.