Figure 2. Applying the cortical shell-liquid core model to a confined cell.

(a) Confocal images of the cell shown in Fig. 1. The two images are Lifeact-mCherry (red) and hMYH9-LAP (green) channels of the same cell and z-plane near mid height. Red and green points denote the shape of the cell boundary as determined by cell edge detection. Scale bars, 10 µm. Fluorescence intensities of cell boundary, cytoplasm and background relate typically as 300/60/6. (b) Comparison of measured cell shape and fit theoretical shape. Solid red and green lines represent the vertical cross section of the rotationally symmetric profile of the fit shape. Data points show mean and standard deviation of surface points representing the measured cell shape in one z-plane (red: Lifeact-mCherry, green: hMYH9-LAP).