Skip to main content
. Author manuscript; available in PMC: 2014 Aug 29.
Published in final edited form as: Nature. 2012 Jun 20;486(7403):405–409. doi: 10.1038/nature11154

Figure 3. MAGI3-AKT3 fusion gene.

Figure 3

A. Diagram of balanced translocation between MAGI3 and AKT3.

B. (top) Genomic DNA PCR for AKT3, MAGI3, and both fusion products in tumour (T) and normal (N). (bottom) cDNA PCR of fusion gene in tumour.

C. (above) MAGI3 and AKT3 protein domains; (below) putative fusion protein.

D. Immunoblots of lysates from ZR-75 cells transfected with vector, MAGI3-AKT3 fusion, or AKT1 E17K mutant, grown in low-serum media, for the indicated antibodies. (Left) infected cells with and without insulin growth factor 1 (IGF-1) stimulation; (right) treatment of vector or MAGI3-AKT3 overexpressing cells with Akt inhibitors MK-2206 and GSK-690693.

E. Focus formation assays with Rat-1 cells expressing pLX control or MAGI3-AKT3, and stained with crystal violet.