Fig. 2.

VP1391 and VP1407 are required for T6SS1 anti-bacterial activity. Viability counts of E. coli (prey) before (0 h) and after (4 h) co-culture. E. coli were co-cultured with V. parahaemolyticus (attacker) POR1 or the following POR1 derivative strains, POR1Δhcp1 and POR1Δvp1391, or POR1Δvp1407, carrying an empty vector or a vector encoding the arabinose-inducible expression of vp1391 (pVP1391) or the expression of vp1407 from its endogenous promoter (pVP1407). Mixed cultures at a 4 : 1 OD₆₀₀ ratio (attacker : prey) were spotted on MLB plates containing 0.1 % (w/v) arabinose and incubated at 30 °C for 4 h.