Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Sep;160(Pt 9):2045–2052. doi: 10.1099/mic.0.081612-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Fig. 1. — Kinetics of myoglobin deoxygenation resulting from oxygen consumption by cytoplasmic membrane preparation. (a) A typical time course of the absorbance difference at 575/560 nm with Zm6 membranes; A_oxy is the absorbance difference recorded immediately after addition of membranes into the assay with oxygenated myoglobin; A_red is the absorbance difference reached after deoxygenation of myoglobin and addition of a small amount of reductant (dithionite). (b) The absorbance differences [between the two vertical bars in (a)] were taken for calculations and for building of Lineweaver–Burk plots of oxygen consumption rate versus the free oxygen concentration, used to find the K_m values (here the calculated K_m value is 1.24 µM).