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. 2014 Sep;160(Pt 9):2045–2052. doi: 10.1099/mic.0.081612-0

Table 1. Plasmids and strains used in the study.

Plasmid/strain Characteristics Source
pGEM-3Zf(+) Ampr Promega
pBT Cmr Stratagene
pGEMperC pGEM-3Zf(+) derivative, carrying a 1.34 kb fragment of PCR-amplified genomic DNA with the ORF of the cytochrome c peroxidase gene (perC; ZZ60192) cloned between the BamHI and HindIII sites of the MCS Present work
pGEMperC : : cmr pGEMperC derivative, carrying in the AgeI site of the perC a 1.3 kb AgeI restriction fragment of pBT, with a 0.7 kb chloramphenicol-resistance ORF Present work
pBBR1MCS-2 Kanr NCBI GenBank
U23751
pBBRperC pBBR1MCS-2 derivative, carrying a 1.57 kb fragment of PCR-amplified genomic DNA with the cytochrome c peroxidase gene (perC; ZZ60192) and its promoter region, cloned between HindIII and BamHI sites of the MCS Present work
Zm6 Parent strain ATCC 29191
Zm6-cytB Zm6 strain with a Cmr insert in the ORF of the cytochrome b subunit gene (ZMO 0957) of the bc1 complex Strazdina et al. (2012)
Zm6-perC Zm6 strain with a Cmr insert in the ORF of perC Present work
Zm6-perCpBBRperC Zm6-perC, carrying plasmid pBBRperC Present work