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. Author manuscript; available in PMC: 2014 Aug 29.
Published in final edited form as: Biomaterials. 2009 Dec 21;31(9):2665–2672. doi: 10.1016/j.biomaterials.2009.12.005

Figure 2. Prolonged perfusion increases gene expression in mouse nasal epithelium.

Figure 2

Mouse nasal epithelium was perfused with pCIKlux (80 μg/mouse) complexed to GL67A or remained untransfected (UT). Carboxymethylcellulose (CMC) to a final concentration of 0.5% was added to the lipid/DNA complexes. To increase contact time between epithelium and gene transfer agents the lipid/DNA complexes were either administered in 150 μl or 400 μl total volume and were then perfused onto the nasal epithelium (7 μl/min) for approximately 23 and 60 min, respectively. 24 hr after transfection luciferase expression was quantified in tissue homogenate. Bars represent group mean ±SEM (n=8/group). ***=p<0.005 compared to 150 μl group.