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. Author manuscript; available in PMC: 2014 Aug 29.
Published in final edited form as: Biomaterials. 2009 Dec 21;31(9):2665–2672. doi: 10.1016/j.biomaterials.2009.12.005

Figure 4. Expression of vector-specific CFTR mRNA in mouse nasal epithelium.

Figure 4

The nasal epithelium of CF knockout mice was perfused with pCIKCFTR or pCIKGFPCFTR (80 μg DNA/mouse) complexed to GL67A including 0.5% CMC (final volume 150 μl/mouse) or remained untransfected (UT). 24 hr after transfection vector-specific and endogenous CFTR mRNA were quantified in nasal epithelium using real-time quantitative RT-PCR. Data are expressed as the ratio of vector-specific over endogenous CFTR mRNA. Each symbol represents one mouse (n=35/group). Horizontal bars indicates group median. In some samples mRNA was detectable, but not accurately quantifiable. These samples are labelled positive (pos). *=p<0.05 compared to pCIKCFTR, ***=p<0.001 compared to untransfected.