Fig. 6.

Oxidative half-reaction of AmPDH variant H103Y followed by stopped-flow spectroscopy. (A) Spectral changes of oxidation of 60 μM AmPDH variant H103Y by 200 μM ferrocenium hexafluorophosphate at 30 °C. Inset: reduced state (a), oxidized state (b) and spectrum observed after prolonged measurement of 50 s (c). (B) Typical time trace and fit at 462 nm of the reaction between 21 μM AmPDH variant H103Y with 100 μM ferrocenium hexafluorophosphate at 30 °C. (C) Plot of pseudo-first-order rate constants versus ferrocenium concentration.