Figure 3.

BMT reduced the oxidative stress response and prevented injury to Hmox1^−/− kidneys. mRNA levels of oxidative stress responsive genes were significantly elevated in KO Ctr animals, including Gsta2 (glutamine-S-transferase A2) (A), Gstm1 (glutamine-S-transferase μ 1) (B), Gclc (glutamate-cysteine ligase, catalytic subunit) (C), Nqo1 [NAD(P)H:quinone dehydrogenase, quinone 1] (D), Fpn1 (ferroportin 1) (E), and Mrp2 (F), but returned to normal after BMT. (G) Masson’s trichrome staining of paraffin-embedded kidney tissues revealed significant accumulation of collagen, which is colored blue (arrows) in KO Ctr mice. Collagen depositions were minimal in transplanted animals. Scale bar represents 50 μm.