TABLE 3.
Plasmids used in this studya
| Name | Description |
|---|---|
| pRS424TEF-MEL1 | TRP1 2μ, for expression of Mel1 from constitutive TEF2 promoter |
| pRS424TEF-MEL1-RFP | TRP1 2μ, for expression of Mel1-RFP from constitutive TEF2 promoter |
| pRS424TEF-spRFP | TRP1 2μ, for expression of mRFP fused to the signal peptide from constitutive TEF2 promoter |
| pRS426-SPO20pr-SUC2 | URA3 2μ, for expression of Suc2 from sporulation specific SPO20 promoter |
| YEp352GAP-II-SUC2 | URA3 2μ, for expression of Suc2 from constitutive TDH2 promoter |
| pRS424TEF-MEL1-3HA | TRP1 2μ, for expression of Mel1-3×HA from constitutive TEF2 promoter |
a
All plasmids originated from this study.