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. 2014 Aug;80(15):4511–4518. doi: 10.1128/AEM.00687-14

FIG 3.

FIG 3

RT-PCR analysis of the transcription of CHU_1277 and the surrounding genes. (A) Illustration of CHU_1277 and the surrounding genes; (B) RT-PCR analysis of the transcription of CHU_1277 and the surrounding genes in the wild-type (WT) strain of C. hutchinsonii and the CHU_1277-disrupted mutant (Δ1277). Lanes 1 to 4, PCRs performed with WT cDNA; lanes 5 to 8, PCRs performed with cDNA of the CHU_1277 mutant; lane 9, marker. The following primers were used for PCRs: 1276RT-1 and 1276RT-2 for lanes 1 and 5; 1277RT-1 and 1277RT-2 for lanes 2 and 6; 1278RT-1 and 1278RT-2 for lanes 3 and 7; 1279RT-1 and 1279RT-2 for lanes 4 and 8.