TABLE 4.
LAMP detection of X. oryzae pv. oyrzae and X. oryzae pv. oryzicola in leaf tissue inoculated by syringe infiltration at 72 hpic
| Strain or samplea | No. of samples positive/total no. of samples amplified with primer set specific for: |
|||
|---|---|---|---|---|
| Pathovar-specific X. oryzae pv. oryzae | Pathovar-specific X. oryzae pv. oryzicola | African lineage of X. oryzae pv. oryzae | Asian lineage of X. oryzae pv. oryzae | |
| PXO99A | 3/3 | 0/3 | 0/3 | 3/3 |
| MAI1 | 3/3 | 0/3 | 3/3 | 0/3 |
| BLS256 | 0/3 | 3/3 | 0/3 | 0/3 |
| Uninoculated leaf | 0/3 | 0/3 | 0/3 | 0/3 |
| No-template controlb | — | — | — | — |
a
The strains are referenced in Table 1.
b
No-template controls contained water and were included in each reaction. —, the absence of any amplification signal.
c
Three independently inoculated leaves were analyzed at least twice.