TABLE 1.
Genetic diversity and frequency distribution of 130 C. jejuni isolates from humans, peas, and wild birds from the outbreake,f
| Sequence type | Clonal complexa | MLST allelic profileb |
PFGE profilec |
Penner serotyped | No. of isolates |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Human |
Wild bird | Pea | Total | |||||||||||||
| aspA | glnA | gltA | glyA | pgm | tkt | uncA | SmaI | KpnI | Outbreak | Sporadic | ||||||
| Human, pea, and wild bird clusters | ||||||||||||||||
| ST-5061* (cluster A) | UA | 10 | 387‡ | 61 | 423 | 39 | 449‡ | 1 | DBRS 16.1283 | DBRK02.0549 | HS19 | 1 | 1 | 2 | ||
| ST-5059* (cluster B) | UA | 10 | 31 | 25 | 49 | 57 | 7 | 313 | DBRS 16.0127 | DBRK02.0546 | HS8 | 5 | 1 | 1 | 7 | |
| ST-5048* (cluster C) | UA | 10 | 383 | 106 | 419‡ | 57 | 443‡ | 1 | DBRS 16.1256 | DBRK02.0522 | HS18 | 3 | 4 | 7 | ||
| ST-137 (cluster D) | ST-45 | 4 | 7 | 10 | 4 | 42 | 7 | 1 | DBRS 16.0749 | DBRK02.0524 | HS15 | 5 | 4 | 9 | ||
| ST-5049* (cluster E) | UA | 180 | 172 | 322 | 47 | 39 | 180 | 3 | Unrestricted | DBRK02.0548 | HS29, HS21 | 2 | 2 | 3 | 7 | |
| Pea and wild bird isolates | ||||||||||||||||
| ST-5047* | UA | 180 | 389 | 95 | 47 | 541 | 442 | 3 | 5 | 4 | 9 | |||||
| ST-5057* | UA | 1 | 389 | 325 | 422 | 541 | 180 | 6 | 4 | 5 | 9 | |||||
| ST-5050* | UA | 178 | 7 | 95 | 6 | 354 | 88 | 1 | 6 | 6 | ||||||
| ST-5056* | UA | 178 | 385 | 95 | 129 | 545 | 180 | 48 | 6 | 6 | ||||||
| ST-5052* | UA | 1 | 31 | 323 | 420 | 394 | 89 | 6 | 4 | 1 | 5 | |||||
| ST-5055* | UA | 10 | 280 | 277 | 49 | 394 | 443‡ | 6 | 5 | 5 | ||||||
| ST-5053* | UA | 1 | 2 | 95 | 62 | 472 | 400 | 147 | 1 | 3 | 4 | |||||
| ST-5054* | UA | 285 | 384 | 324 | 421 | 542 | 445 | 312 | 3 | 3 | ||||||
| ST-5073* | UA | 178 | 8 | 327 | 49 | 544 | 28 | 6 | 3 | 3 | ||||||
| ST-5058* | UA | 1 | 2 | 34 | 90 | 543 | 443‡ | 6 | 1 | 1 | 2 | |||||
| ST-5051* | UA | 10 | 385 | 106 | 129 | 39 | 444 | 3 | 1 | 1 | ||||||
| ST-5060* | UA | 10 | 387‡ | 95 | 423 | 39 | 7 | 1 | 1 | 1 | ||||||
| ST-5072* | UA | 290 | 389 | 5 | 418 | 39 | 441 | 6 | 1 | 1 | ||||||
| ST-5074* | UA | 10 | 386 | 61 | 419‡ | 39 | 451 | 1 | 1 | 1 | ||||||
| Human outbreak-associated isolates | ||||||||||||||||
| ST-5062*† | UA | 287 | 6 | 34 | 424 | 394 | 446 | 172 | HS41 | 8 | 9 | |||||
| HS8 | 1 | |||||||||||||||
| ST-468 | UA | 10 | 2 | 50 | 62 | 91 | 73 | 45 | 3 | 3 | ||||||
| ST-5069* | UA | 10 | 6 | 61 | 427 | 548 | 447 | 6 | 3 | 3 | ||||||
| ST-5063* | UA | 10 | 308 | 106 | 47 | 57 | 447 | 1 | 2 | 2 | ||||||
| ST-5065* | UA | 1 | 172 | 5 | 426 | 39 | 231 | 6 | 2 | 2 | ||||||
| ST-5064* | UA | 1 | 82 | 95 | 425 | 546 | 448 | 314 | 1 | 1 | ||||||
| ST-5067* | UA | 286 | 172 | 5 | 426 | 39 | 443‡ | 6 | 1 | 1 | ||||||
| ST-5066* | UA | 10 | 74 | 276 | 47 | 39 | 449‡ | 51 | 2 | 2 | ||||||
| ST-45 | ST-45 | 4 | 7 | 10 | 4 | 1 | 7 | 1 | 2 | 2 | ||||||
| ST-42 | ST-42 | 1 | 2 | 3 | 4 | 5 | 9 | 3 | 1 | 1 | ||||||
| ST-48 | ST-48 | 2 | 4 | 1 | 2 | 7 | 1 | 5 | 1 | 1 | ||||||
| Human sporadic isolates | ||||||||||||||||
| ST-5066* | UA | 10 | 74 | 276 | 47 | 39 | 449‡ | 51 | 1 | 1 | ||||||
| ST-45 | ST-45 | 4 | 7 | 10 | 4 | 1 | 7 | 1 | 3 | 3 | ||||||
| ST-42 | ST-42 | 1 | 2 | 3 | 4 | 5 | 9 | 3 | 1 | 1 | ||||||
| ST-48 | ST-48 | 2 | 4 | 1 | 2 | 7 | 1 | 5 | 1 | 1 | ||||||
| ST-19 | ST-21 | 2 | 1 | 5 | 3 | 2 | 1 | 5 | 1 | 1 | ||||||
| ST-52 | ST-52 | 9 | 25 | 2 | 10 | 22 | 3 | 6 | 1 | 1 | ||||||
| ST-353 | ST-353 | 7 | 17 | 5 | 2 | 10 | 3 | 6 | 1 | 1 | ||||||
| ST-403 | ST-403 | 10 | 27 | 16 | 19 | 10 | 5 | 7 | 1 | 1 | ||||||
| ST-508 | ST-508 | 1 | 6 | 60 | 24 | 12 | 28 | 1 | 1 | 1 | ||||||
| ST-572 | ST-206 | 62 | 4 | 5 | 2 | 2 | 1 | 5 | 1 | 1 | ||||||
| ST-991 | ST-692 | 37 | 52 | 57 | 26 | 107 | 29 | 23 | 1 | 1 | ||||||
| ST-2875 | UA | 2 | 29 | 4 | 48 | 10 | 25 | 57 | 1 | 1 | ||||||
| ST-5068* | ST-42 | 1 | 2 | 3 | 4 | 5 | 9 | 315 | 1 | 1 | ||||||
| Total | 43 | 16 | 31 | 40 | 130 | |||||||||||
UA, unassigned status for a given sequence type.
Newly identified alleles are shown in bold.
PFGE profiles for the restriction enzymes SmaI and KpnI for the five identified clusters (A to E).
Penner serotyping was performed on representatives of human and environmental isolates from clusters A to E and on all ST-5062 isolates, including one from a GBS case.
*, novel STs; †, ST associated with one case of Guillain-Barré syndrome; ‡, overlapping novel alleles found in human and environmental isolates.
The numbers of alleles per locus (% novel) were 14 (28.5), 23 (26), 22 (22.7), 24 (41.6), 22 (31.8), 24 (41.6), and 16 (25) for the aspA, glnA, gltA, glyA, pgm, tkt, and uncA genes, respectively.