TABLE 4.

Reported consensus sequences flanking the invariant catalytic triad residues of nitrilases^a

Conserved catalytic triad of nitrilases	Consensus sequence	Nitrilase sequence alignment (this study)
E44	f P E a f	m P E A l
K131	h R K l * p T	H R K L M P T
C165	l * C W E n ** p	a i C W E N * M P

^aReported consensus sequences flanking the invariant catalytic triad residues of nitrilases as reported by Pace and Brenner (24) and the consensus sequences flanking the catalytic triad from the nitrilase multiple sequence alignment in this study. Uppercase letters indicate ≥90% consensus, whereas lowercase letters represent ≥50% consensus. An asterisk (*) represents residues with <50% consensus. Boldface indicates residues of the catalytic triad (E-K-C).