TABLE 5.
Further analysis of regions flanking the conserved catalytic triad of the nitrilase multiple sequence alignmenta
| Conserved catalytic triad of nitrilases | Nitrilase sequence alignment (this study) |
|---|---|
| E44 | LVV* PEA * |
| LGGYPKG | |
| K131 | HRKLMPT ** ERLIWG |
| *GDGST | |
| C165 | CWEN * MPL |
| LR* AMY |
a
Further analysis of regions flanking the conserved catalytic triad of the nitrilase multiple sequence alignment in the present study. Underlined residues were 100% conserved over all of the nitrilase sequences, and residues that are not underlined have at least 90% consensus. An asterisk (*) indicates residues with <90% consensus. Boldface indicates residues of the catalytic triad (E-K-C).