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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Nat Immunol. 2014 Aug 10;15(9):875–883. doi: 10.1038/ni.2958

Figure 7.

Figure 7

Elevated LFA-1-dependent adhesion and conjugate formation by Ptpn22−/− T cells is important for the increased responses to weak Ags. (a) Flow cytometry analyses assessing conjugate formation of naive WT and Ptpn22−/− Rag1−/− OT-1 T cells with N4, T4, G4 or SIY-loaded APCs. Error bars represent SD (n=3 replicate samples) and data are representative of 3 repeated experiments. (b) Blocking LFA-1 mAbs were added at various time points, as indicated, following N4 or G4 peptide stimulation of naive WT and Ptpn22−/− OT-1 cells and CD69 upregulation measured at 4 h. Data shown are means ± SD (n=3 replicate samples) from one of two repeated experiments. (c) Adhesion of WT and KO effector CTL to ICAM-1 under flow. Error bars represent SD (n=4 mice/group) and data are representative of 2 repeated experiments. NS – not significant, * p<0.05, ** p<0.01, *** p<0.001 by Student’s t-test (a) or ANOVA (c).