FIGURE 3.

GPR107-deficient KBM7 cells are resistant to PE and C. jejuni CDT but sensitive to the other CDTs. A, map of unique insertion sites in the GPR107 gene in the surviving KBM7 cell population. Boxes denote transmembrane domains. B, RT-PCR analysis showing the absence of GPR107 transcript in the clonal cell line carrying gene-trap insertions in the GPR107 gene. C, KBM7^WT, GPR107^GT, and GPR107^GT cells reconstituted with GPR107 cDNA were intoxicated with PE; cell viability was determined and shown as percent relative to nonintoxicated cells. Error bars represent S.D. of three experiments performed in duplicate. D, cells were intoxicated for 18 h with CDTs of different bacterial origin, stained with propidium iodide, and subjected to FACS analysis to assess the G₂/M cell cycle arrest. Intensity of fluorescence was measured, and the percent maximum was presented in the overlaid histograms.