FIGURE 8.

GPR107 is essential for retrograde trafficking of cholera toxin. A, equal numbers of KBM7^WT, GPR107^GT, and reconstituted GPR107^GT cells were intoxicated with CTx-GGGK(biotin)-NNSTG for 30 min and chased for the indicated time points. Cells were lysed, and the glycosylation of CTx was analyzed by Western blotting using streptavidin-HRP. B, quantification of experiments performed as described in A. C, schematic representation of the intracellular pathway of PE based on the results of the genetic screen. Novel genes identified in the screen are shown in red.