FIGURE 9.

Silencing of CD44, EphA2, Src, PATJ, or DBS reduces RhoA activation in response to LMW-HA and inhibits HLMVEC migration. Panel A, ECs that had been subjected to control siRNA or target protein siRNA were incubated ± LMW-HA (100 nm) for 15 min. Total cell lysates were then prepared, and a pulldown assay for GTP-bound active RhoA was performed. Panel B, ECs were incubated with the indicated siRNAs and then plated on 8-μm transwell filters in serum-free media. 10% serum media was added to the bottom wells, and cells were incubated for 24 h. Cell numbers from the upper and lower chambers were quantified using the MTS cell assay. An asterisk (*) indicates a statistically significant difference (p < 0.05) from control and scramble siRNA. n = 3 per condition.