TABLE 2.

Kinetic parameters of LpxK point mutants with respect to DSMP

LpxK mutant	Apparent Km	Km increase	Apparent kcat	kcat decrease
	μm	-fold	s−1	-fold
WT	7 ± 1	1	2.4 ± 0.1	1
N43A	9 ± 2	1	(3.6 ± 0.3) ×10−1	6.7
R72A	28 ± 3	4	(3.9 ± 0.1) ×10−1	6.2
Y74A	48 ± 7	7	(3.1 ± 0.2) ×10−2	77
E100A	17.4 ± 0.8	3	(1.23 ± 0.02) ×10−3	2,000
R119A	9.7 ± 0.6	1	(3.60 ± 0.07) ×10−2	67
D138A	47 ± 5	7	(1.06 ± 0.05) ×10−3	2,300
D138N	7 ± 2	1	(2.8 ± 0.2) ×10−2	87
D139A	46 ± 8	7	(5.1 ± 0.4) ×10−4	4,700
D139N	14 ± 2	2	(6.3 ± 0.2) ×10−3	380
Q142A	28 ± 3	4	(3.5 ± 0.1) ×10−1	6.8
H143A	50 ± 10	7	(1.6 ± 0.2) ×10−1	15
R171A	128 ± 9	20	(1.38 ± 0.06) ×10−2	170
E172A	18 ± 3	3	(7.0 ± 0.1) ×10−1	3.4
H261A	15 ± 1	2	(3.1 ± 0.9) ×10−3	770