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. 2014 Jun 25;289(35):24250–24262. doi: 10.1074/jbc.M114.553644

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — FRET experiments analyzing CRF₁R dimerization specificity in transiently transfected HEK 293 cells. A, FRET spectra (± S.D.) were recorded for cells expressing CRF₁R.CFP alone (black, n = 73) or the CRF₁R.YFP/CRF₁R.CFP FRET pair (red, n = 120). In addition, cells expressing CRF₁R.YFP/CRF₁R.CFP were co-transfected with the untagged CRF₁R (green, n = 95) or V₂R (blue, n = 140). B, energy transfer efficiencies (E_T% ± S.D.) calculated out of A. E_T values were derived as described previously (14). n.s., not significant; ***, highly significant.