TABLE 3.
FCCS fitting parameters (± S.E.) for the GFP- and mCherry-tagged CRF1R and ETBR in the ER membrane of transiently transfected HEK 293 cells
Decay times (τD, 1 (ms); τD, 2 (ms); fractions (τD, 1 (%); τD, 2 (%)), and CC values (G(0)x/G(0)min) are listed. The best fits were obtained assuming free diffusion in three dimensions and two components (3D_2C). Triplet fractions (TF (%)) ranged from 20 to 50% and triplet decay times (τD,F (μs)) from 20 to 200 μs.
| GPCRs | τD, 1 | τD, 2 | τD, 1 | τD, 1 | N | G(0)x/G(0)min |
|---|---|---|---|---|---|---|
| % | % | ms | ms | % | ||
| CRF1R | ||||||
| GFP | 56 ± 1 | 44 ± 1 | 0.37 ± 0.02 | 34 ± 1 | 89 | 17.8 ± 0.7 |
| mCherry | 61 ± 1 | 39 ± 1 | 0.20 ± 0.04 | 28 ± 2 | ||
| ETBR | ||||||
| GFP | 34 ± 1 | 66 ± 1 | 0.62 ± 0.04 | 51 ± 2 | 77 | 14.6 ± 0.7 |
| mCherry | 62 ± 1 | 38 ± 1 | 0.13 ± 0.01 | 45 ± 3 | ||