FIGURE 3.

SAXS and SEC data confirm the quaternary structure observed from crystallographic data. A, ACOT12 scattering data (red dots) superimposed on the theoretical scattering curves (blue line) for an ACOT12 monomer, dimer, trimer, and tetramer using CRYSOL with χ values of 62, 25, 1.3, and 14.4, respectively. B, ACOT12-ADP scattering data with the theoretical scattering curves for ACOT12-ADP monomer, dimer, trimer, and tetramer superimposed with χ values of 25, 7, 1.8, and 5.5. C, SEC elution profiles for apo-ACOT12, ACOT12-ADP, and ACOT12-ATP plotted against known molecular weight standards to assess the native molecular weight demonstrate that neither ADP nor ATP disrupt oligomerization, consistent with the SAXS and crystallographic data.