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Overexpression of FLAG-VIMP causes ER-MT bundling. A, amino acid sequence of VIMP. Amino acid residues in the putative transmembrane domain are shown in blue. U represents the penultimate selenocysteine. B, at 24 h after transfection with the FLAG-VIMP(1–187) construct, HeLa cells were fixed and double-stained with antibodies against FLAG and calnexin (upper panels) or α-tubulin (lower panels). Scale bar = 5 μm.
