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VIMP does not interact with smooth ER-shaping proteins. Lysates of 293T cells expressing FLAG-tagged wild-type Syn5L or mutant K82A (A); Reep1-FLAG, Reep5-FLAG, FLAG-ATL1, or FLAG-tagged wild-type Syn5L (B); or FLAG-tagged wild-type Syn5L or one of the FLAG-RTN proteins (C) were subjected to immunoprecipitation (IP) with anti-FLAG M2 beads and analyzed by immunoblotting with antibodies against VIMP and FLAG. Oligomer (*) and monomer (**) species are indicated.
