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. 2014 Jul 9;289(35):24304–24313. doi: 10.1074/jbc.M114.571372

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Localization of VIMP. A, lysates of HeLa cells were subjected to fractionation using a 5–25% OptiPrep gradient. Fractions (0.3 ml each) were collected from the top of the gradient. Appropriate portions of each fraction were subjected to SDS-PAGE and analyzed by immunoblotting with antibodies against Golgi marker GM130 (upper panel), RTN4 (second panel), CLIMP-63 (third panel), and VIMP (lower panel). B, immunoelectron micrograph. Arrowheads and arrows indicate ER membranes and immunoreactivity to VIMP, respectively. N and M denote nucleus and mitochondria, respectively. Scale bar = 0.5 μm.