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. 2014 Jul 11;289(35):24336–24346. doi: 10.1074/jbc.M114.588038

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 9. — In vivo oligomerization of Pex5p is impaired in pex1 ZP107 cells. A, cytosolic fractions each from CHO-K1 (lane 1), pex14 ZP161 (lane 2), and pex1 ZP107 (lane 3) were separated by blue native PAGE. Protein complexes were detected by immunoblotting with anti-Pex5p antibody. Molecular mass markers are on the left. BN, blue native; WB, Western blot. B, purified recombinant His-Pex5p-FLAG was introduced into CHO-K1 (lane 1), pex14 ZP161 (lane 2), pex1 ZP107 (lane 3), pex6 ZP164 (lane 4), pex26 ZP167 (lane 5), and pex5 ZP101 (lane 6) cells. Cells were then cultured for 24 h at 37 °C. His-Pex5p-FLAG was immunoprecipitated from cell lysates using anti-FLAG antibody. Immunoprecipitates were analyzed by SDS-PAGE and immunoblotting with anti-Pex5p antibody. Input, 10% input. Solid and open arrowheads indicate endogenous Pex5p and His-Pex5p-FLAG, respectively. *, a nonspecific band.