FIGURE 4.

Minimal effects of Dim1 on the expression of c-Fos and NF-κB p65. A, total RNA was prepared from mock- or Dim1-depleted BMMs after treating with M-CSF and RANKL for 0, 1, 2, and 3 days. The mRNA levels of c-fos, NF-κB p65, and Nfatc1 genes were quantified by qRT-PCR. B, qRT-PCR was performed as described in A, but using total RNA isolated from Dim1 expression virus- or control virus-infected BMM cells. C, RAW 264.7 macrophage cells were transiently transfected with c-Fos, NF-κB p65, or NFATc1 reporter together with Dim1 expression vector. 24 h post-transfection, cells were treated with RANKL (50 ng/ml) for 16 h, and luciferase activity was measured using Dual-Luciferase assay kit (Promega). Each bar represents the means ± S.D. of three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001.